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| Registros recuperados: 10 | |
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| Mugnier, Chantal; Justou, Carole; Lemonnier, Hugues; Patrois, Jacques; Ansquer, Dominique; Goarant, Cyrille; Le Coz, Jean-rene. |
| Shrimp aquaculture in New Caledonia is subject to seasonal mortalities during grow-out due to highly virulent Vibrio nigripulchritudo (Vn). To understand the mechanisms affecting shrimp resistance and leading to significant mortality, a shrimp ecophysiology and immunology survey was conducted on two farms, the first considered as a "control" farm (HC), the second affected by the disease (DF). Mortality observed during the survey at DF started 50 days after stocking and was typical of this disease. The main observations regarding shrimp were: (a) growth was not affected by the disease and was faster in the DF than in the HC pond; (b) disease did not affect one sex more than the other, or a specific part of the population in terms of weight; (c) the physical... |
| Tipo: Text |
Palavras-chave: Vibriosis; Penaeid shrimp; Field survey; Physiology; Immunology; Nutritional status. |
| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00176/28764/27567.pdf |
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| Pilotto, Mariana R.; Goncalves, Andre N. A.; Vieira, Felipe N.; Seifert, Walter Q.; Bachere, Evelyne; Rosa, Rafael D.; Perazzolo, Luciane M.. |
| We provide a global overview of the intestinal bacteriome of Litopenaeus vannamei in two rearing systems and after an oral challenge by the White spot syndrome virus (WSSV). By using a high-throughput 16S rRNA gene sequencing technology, we identified and compared the composition and abundance of bacterial communities from the midgut of shrimp reared in the super-intensive biofloc technology (BFT) and clear seawater system (CWS). The predominant bacterial group belonged to the phylum Proteobacteria, followed by the phyla Bacteroidetes, Actinobacteria, and Firmicutes. Within Proteobacteria, the family Vibrionaceae, which includes opportunistic shrimp pathogens, was more abundant in CWS than in BFT-reared shrimp. Whereas the families Rhodobacteraceae and... |
| Tipo: Text |
Palavras-chave: Biofloc technology (BFT); Penaeid shrimp; Gut microbiota; White spot syndrome virus; 16S rRNA gene sequencing. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00454/56546/58256.pdf |
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| Farias, Natanael Dantas; Falchetti, Marcelo; Matos, Gabriel Machado; Schmitt, Paulina; Barreto, Cairé; Argenta, Nicolas; Rolland, Jean-luc; Bachère, Evelyne; Perazzolo, Luciane Maria; Rosa, Rafael Diego. |
| Stylicins are anionic antimicrobial host defense peptides (AAMPs) composed of a proline-rich N-terminal region and a C-terminal portion containing 13 conserved cysteine residues. Here, we have increased our knowledge about these unexplored crustacean AAMPs by the characterization of novel stylicin members in the most cultivated penaeid shrimp, Litopenaeus vannamei. We showed that the L. vannamei stylicin family is composed of two members (Lvan-Stylicin1 and Lvan-Stylicin2) encoded by different loci which vary in gene copy number. Unlike the other three gene-encoded antimicrobial peptide families from penaeid shrimp, the expression of Lvan-Stylicins is not restricted to hemocytes. Indeed, they are also produced by the columnar epithelial cells lining the... |
| Tipo: Text |
Palavras-chave: Crustacean; Penaeid shrimp; Invertebrate immunity; Antimicrobial peptide; Host defense peptide; Molecular diversity. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00466/57816/60102.pdf |
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| Braunig,Patricia; Rosa,Rafael Diego da; Seibert,Caroline Heidrich; Borsa,Mariana; Stoco,Patricia Hermes; Grisard,Edmundo Carlos; Pinto,Aguinaldo Roberto. |
| In the present study, a fragment of the VP28 coding sequence from a Brazilian WSSV isolate (BrVP28) was cloned, sequenced and expressed in E. coli BL21(DE3) pLysS strain in order to produce the VP28 carboxyl-terminal hydrophilic region. The expression resulted in a protein of about 21 kDa, which was purified under denaturing conditions, resulting in a final highly purified BrVP28 preparation. The recombinant protein obtained can be used in several biotechnology applications, such as the production of monoclonal antibodies which could be used in the development of diagnostic tools as well as in the studies on the characterization of white spot syndrome virus (WSSV) isolated in Brazil. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: WSSV; VP28; Recombinant protein; Penaeid shrimp. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000200023 |
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| Destoumieux, Delphine; Munoz, Marcello; Cosseau, Céline; Rodriguez, Jenny; Bulet, Philippe; Comps, Marie-annick; Bachere, Evelyne. |
| Penaeidins are members of a new family of antimicrobial peptides isolated from a crustacean, which present both Gram-positive antibacterial and antifungal activities. We have studied the localization of synthesis and storage of penaeidins in the shrimp Penaeus vannamei. The distribution of penaeidin transcripts and peptides in various tissues reveals that penaeidins are constitutively synthesized and stored in the shrimp haemocytes. It was shown by immunocytochemistry, at both optical and ultrastructural levels, that the peptides are localized in granulocyte cytoplasmic granules, The expression and localization of penaeidins were further analysed in shrimp subjected to microbial challenge. We found that (1) penaeidin mRNA levels decrease in circulating... |
| Tipo: Text |
Palavras-chave: Crustacean immunity; Penaeid shrimp; Haemocyte; Chitin binding; Antimicrobial peptide. |
| Ano: 2000 |
URL: http://archimer.ifremer.fr/doc/2000/publication-412.pdf |
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| Gueguen, Yannick; Garnier, Julien; Robert, Lorenne; Lefranc, Marie-paule; Mougenot, Isabelle; De Lorgeril, Julien; Janech, Michael; Gross, Paul S.; Warr, Gregory W.; Cuthbertson, Brandon; Barracco, Margherita A.; Bulet, Philippe; Aumelas, André; Yang, Yinshan; Bo, Dong; Xiang, Jianhai; Tassanakajon, Anchalee; Piquemal, Piquemal; Bachere, Evelyne. |
| Antimicrobial peptides play a major role in innate immunity. The penaeidins, initially characterized from the shrimp Litopenaeus vannamei, are a family of antimicrobial peptides that appear to be expressed in all penaeid shrimps. As of recent, a large number of penaeid nucleotide sequences have been identified from a variety of penaeid shrimp species and these sequences currently reside in several databases under unique identifiers with no nomenclatural continuity. To facilitate research in this field and avoid potential confusion due to a diverse number of nomenclatural designations, we have made a systematic effort to collect, analyse, and classify all the penaeidin sequences available in every database. We have identified a common penaeidin signature... |
| Tipo: Text |
Palavras-chave: Nomenclature; Sequence database; Crustacea; Antimicrobial peptide; Penaeid shrimp; Penaeidin. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-2332.pdf |
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| Mugnier, Chantal; Lemonnier, Hugues; Legrand, Alexandre. |
| The blue shrimp Litopenaeus stylirostris was exposed to pond sediment for 48 h and its physiological response was studied in relation to molt stage. Shrimp were maintained in cages within a semi-intensive pond after 4 months of rearing. Hypoosmoregulatory capacity (hypo-OC), magnesium ions (Mg ions), glucose, total proteins and oxyhaemocyanin were assayed in haemolymph. An increase of 370% to 500% in Mg ions concentration and 200% to 266% in glucose concentration, and a decrease in hypo-OC in shrimp maintained close to the centre of the pond indicated a short-term stress response in all molt stages. Indicators of water and sediment pore water quality were within normal operating limits, however this area was characterized by high water content and... |
| Tipo: Text |
Palavras-chave: Stress; Pond bottom; Penaeid shrimp; Molt stage; Haemolymph constituents; Litopenaeus stylirostris. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-1675.pdf |
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| Mugnier, Chantal; Soyez, Claude. |
| The effect of different levels of dissolved oxygen (DO) and a decreased temperature combined with severe hypoxia were studied in the blue shrimp Litopenaeus stylirostris in relation to molt stage. In the first experiment, shrimps were submitted to DO concentrations of 5 to 1 mg l(-1). Osmoregulatory capacity (OC) was measured in shrimps at molt stage C after 6, 24, or 48 h of exposure. No mortality was recorded but a significant negative effect of DO concentration on hypo-OC was observed for DO concentrations below 3 mg l(-1). Osmoregulation improved after 48 h exposure, compared to 6 and 24 h exposure. In a second experiment, temperature was decreased from 28 to 22 degreesC in 24 h, before a severe hypoxia of 0.5+/-0.1 mg O-2 l(-1) was applied.... |
| Tipo: Text |
Palavras-chave: Temperature; Stress; Penaeid shrimp; Osmoregulation; Molt stage; Litopenaeus stylirostris; Hypoxia. |
| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/publication-372.pdf |
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| Mari, Jocelyne; Bonami, Jean-robert; Lightner, Donald V. |
| The ssRNA genome extracted from purified Taura Syndrome Virus (TSV) was transcribed into double-stranded, blunt-ended cDNA and was used to construct cDNA libraries either in pUC 18 or in pBluescript II KS-vectors. Twelve recombinant plasmids chosen after screening of the libraries were subjected to restriction enzyme digestions for determination of size inserts and restriction maps. Two of them, pP15 and pQ1, were selected for probe construction. The inserts, 1500 and 1300 base pairs (bp) respectively, were DIG-11dUTP-labelled and the corresponding probes were named P15 and Q1. On northern blots and dot blots, using different denaturation methods, the 2 probes hybridized specifically with extracted RNA-TSV genome, TSV and infected TS shrimp homogenates. No... |
| Tipo: Text |
Palavras-chave: TSV; Penaeid shrimp; Picornavirus; Cloning; Dot blot hybridization; In situ hybridization. |
| Ano: 1998 |
URL: http://archimer.ifremer.fr/doc/00447/55899/57914.pdf |
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| Registros recuperados: 10 | |
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